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A rapid densitometric method for the analysis of hyoscyamine and scopolamine in solanaceous plants and their transformed root cultures
Author(s) -
Berkov Strahil,
Pavlov Atanas
Publication year - 2004
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.756
Subject(s) - hyoscyamine , chemistry , scopolamine , chromatography , alkaloid , densitometry , biochemistry , solanaceae , pharmacology , stereochemistry , biology , quantum mechanics , gene , physics
A rapid and convenient method for sample preparation and simultaneous densitometric quantication of hyoscyamine (1) and scopolamine (2) has been developed. The alkaloid proles of plant samples, as well as the content of 1 and 2, determined by TLC‐densitometry corresponded well with those obtained using a GC method. The proposed method is simple and sensitive and can be used for the routine assay of 1 and 2, and for screening for alkaloid‐rich and biochemically‐interesting transformed root cultures and intact solanaceous plants. Copyright © 2004 John Wiley & Sons, Ltd.