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Determination of potato glycoalkaloids using high‐pressure liquid chromatography–electrospray ionisation/mass spectrometry
Author(s) -
Matsuda Fumio,
Morino Keiko,
Miyazawa Haruna,
Miyashita Masahiro,
Miyagawa Hisashi
Publication year - 2004
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.755
Subject(s) - chemistry , chromatography , electrospray , electrospray ionization , mass spectrometry , solanum tuberosum , high performance liquid chromatography , glycoalkaloid , electrospray mass spectrometry , acetic acid , solanaceae , biochemistry , botany , gene , biology
A method for quantifying two toxic glycoalkaloids, α ‐solanine and α ‐chaconine, in potato ( Solanum tuberosum ) tuber tissue was developed using HPLC‐electrospray ionisation (ESI)/MS. Potato samples were extracted with 5% aqueous acetic acid, and the extracts were subjected directly to HPLC‐ESI/MS after ltration. By determining the intensities of the protonated molecules of α ‐solanine ( m/z 868) and α ‐chaconine ( m/z 852) using selected ion monitoring (positive ion mode), a sensitive assay was attained with detection limits of 38 and 14 ppb for the two glycoalkaloids, respectively. The high sensitivity and selectivity of MS detection effectively reduced the time of analysis thus enabling a high throughput assay of glycoalkaloids in potato tubers. Copyright © 2004 John Wiley & Sons, Ltd.