Identiﬁcation of the major vanilloid component in  Capsicum  extract by HPLC‐EC and HPLC‐MS | Zendy

Zhou Qin | Zendy; Zhu Yongxin | Zendy; Chiang Hwa | Zendy; Yagiz Kader | Zendy; Morre D. James | Zendy; Morre Dorothy M. | Zendy; Janle Elsa | Zendy; Kissinger Peter T. | Zendy

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Identiﬁcation of the major vanilloid component in Capsicum extract by HPLC‐EC and HPLC‐MS

Author(s) -

Zhou Qin,

Zhu Yongxin,

Chiang Hwa,

Yagiz Kader,

Morre D. James,

Morre Dorothy M.,

Janle Elsa,

Kissinger Peter T.

Publication year - 2004

Publication title -

phytochemical analysis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.574

H-Index - 72

eISSN - 1099-1565

pISSN - 0958-0344

DOI - 10.1002/pca.754

Subject(s) - chemistry , chromatography , vanillic acid , high performance liquid chromatography , acetonitrile , formic acid

A sensitive multi‐channel HPLC‐electrochemical (EC) method has been developed to determine the vanilloid content in the complex Capsicum annuum extract Capsibiol ® . Chromatographic separation was achieved within 10 min using a YMC Basic S5 column with a mobile phase containing chloroacetic acid, heptane sulphonic acid and acetonitrile. The multi‐channel detector simultaneously applied four potentials between +500 and +800 mV (referenced to a silver/silver chloride electrode) to four glassy carbon working electrodes. The most abundant (0.94 mg/g) vanilloid analogue in the Capsibiol sample demonstrated an electrochemical reactivity and retention time similar to that of vanillic acid in HPLC‐EC analysis. Its identity was conrmed by HPLC‐MS using a Zorbax SB‐CN column with a mobile phase containing formic acid and methanol. Copyright © 2004 John Wiley & Sons, Ltd.

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