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Identification of the major vanilloid component in Capsicum extract by HPLC‐EC and HPLC‐MS
Author(s) -
Zhou Qin,
Zhu Yongxin,
Chiang Hwa,
Yagiz Kader,
Morre D. James,
Morre Dorothy M.,
Janle Elsa,
Kissinger Peter T.
Publication year - 2004
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.754
Subject(s) - chemistry , chromatography , vanillic acid , high performance liquid chromatography , acetonitrile , formic acid
A sensitive multi‐channel HPLC‐electrochemical (EC) method has been developed to determine the vanilloid content in the complex Capsicum annuum extract Capsibiol ® . Chromatographic separation was achieved within 10 min using a YMC Basic S5 column with a mobile phase containing chloroacetic acid, heptane sulphonic acid and acetonitrile. The multi‐channel detector simultaneously applied four potentials between +500 and +800 mV (referenced to a silver/silver chloride electrode) to four glassy carbon working electrodes. The most abundant (0.94 mg/g) vanilloid analogue in the Capsibiol sample demonstrated an electrochemical reactivity and retention time similar to that of vanillic acid in HPLC‐EC analysis. Its identity was conrmed by HPLC‐MS using a Zorbax SB‐CN column with a mobile phase containing formic acid and methanol. Copyright © 2004 John Wiley & Sons, Ltd.