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Development of a one‐step immunochromatographic strip test for the detection of sennosides A and B
Author(s) -
Putalun Waraporn,
Morinaga Osamu,
Tanaka Hiroyuki,
Shoyama Yukihiro
Publication year - 2004
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.752
Subject(s) - chemistry , nitrocellulose , reagent , chromatography , conjugate , colloidal gold , analyte , immunoassay , monoclonal antibody , detection limit , membrane , antibody , nanoparticle , biochemistry , nanotechnology , organic chemistry , mathematical analysis , materials science , mathematics , immunology , biology
An immunochromatographic strip test was developed to detect sennoside A (1) and sennoside B (2) using anti‐1 and anti‐2 monoclonal antibodies. The qualitative assay was based on a competitive immunoassay in which the detector reagent consisted of colloidal gold particles coated with the respective sennoside antibodies. The capture reagents were 1‐ and 2‐human serum albumin (HSA) conjugates immobilised on a nitrocellulose membrane on the test strip. The sample containing 1 and 2, together with detector reagent, passed over the zone where the capture reagents had been immobilised. The analytes in the sample competed for binding to the limited amount of antibodies in the detector reagent with the immobilised 1‐ and 2‐HSA conjugates on the membrane and hence positive samples showed no colour in the capture spot zone. Detection limits for the strip test were 125 ng/mL for both sennosides. The assay system is useful as a rapid and simple screening method for the detection of 1 and 2 in plants, drugs and body uids. Copyright © 2004 John Wiley & Sons, Ltd.