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Screening for oestrogenic activity of plant and food extracts using in vitro trout hepatocyte cultures
Author(s) -
BennetauPelissero C.,
Gontier Latonnelle K.,
Lamothe V.,
ShinkarukPoix S.,
Kaushik S. J.
Publication year - 2004
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.741
Subject(s) - chemistry , kudzu , in vitro , hepatocyte , phytosterol , food science , trout , biochemistry , fish <actinopterygii> , biology , fishery , medicine , alternative medicine , pathology , traditional chinese medicine
The use of in vitro trout hepatocyte cultures is shown to provide a simple and effective way to screen plant and food products for oestrogenic activity. The relative oestrogenic activities of 0.1 g each of extracts of phytosterol, soy isoavone, red clover, kudzu and soybean extracts were determined using this assay and found to be equivalent to 212, 1, 3.2, 132 and 1025 n m of 17 β ‐estradiol, respectively. Controls were performed on soybean and kudzu extracts using specic ELISAs for isoavones and these conrmed the validity of the cell culture assay. The method described offers an advantage over current methods in that it can detect increased oestrogenic activity that may occur as a result of metabolic activation of pre‐ or pro‐oestrogens liver cells. Copyright © 2004 John Wiley & Sons, Ltd.