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Identification and quantification of components in extracts of Uncaria tomentosa by HPLC‐ES/MS
Author(s) -
Montoro P.,
Carbone V.,
de Dioz Zuniga Quiroz J.,
De Simone F.,
Pizza C.
Publication year - 2004
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.740
Subject(s) - chemistry , chromatography , glycoside , high performance liquid chromatography , bark (sound) , extraction (chemistry) , solid phase extraction , rubiaceae , botany , organic chemistry , physics , acoustics , biology
The two main classes of secondary metabolites, alkaloids and quinovic acid glycosides, of Uncaria tomentosa (Willd.) DC. (Rubiaceae), a Peruvian plant commonly known as ‘uña de gato’, have been analysed. Separation of the alkaloidal fraction was achieved using a solid phase extraction method based on cationic exchange, and an analytical method employing HPLC‐ES/MS has been developed. Quantitative data for commercial wild bark, cultivated bark and leaves are reported. The analysis of quinovic acid glycosides was performed directly on the crude extract using both a fast analytical method based on ow injection ES/MS, and a more complete analytical technique using HPLC‐MS. Copyright © 2004 John Wiley & Sons, Ltd.
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