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Simultaneous quantitative determination of eight triterpenoid monoesters from flowers of 10 varieties of Calendula officinalis L. and characterisation of a new triterpenoid monoester
Author(s) -
Neukirch Hannes,
D'Ambrosio Michele,
Via Josef Dalla,
Guerriero Antonio
Publication year - 2004
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.739
Subject(s) - chemistry , calendula officinalis , orange (colour) , chromatography , terpenoid , terpene , triterpenoid , dichloromethane , high performance liquid chromatography , botany , stereochemistry , food science , organic chemistry , solvent , biology
Dichloromethane extracts of dried owers of Calendula ofcinalis contain eight known bioactive triterpendiol monoesters, namely, faradiol‐3‐ O ‐palmitate, faradiol‐3‐ O ‐myristate, faradiol‐3‐ O ‐laurate, arnidiol‐3‐ O ‐palmitate, arnidiol‐3‐ O ‐myristate, arnidiol‐3‐ O ‐laurate, calenduladiol‐3‐ O ‐palmitate and calenduladiol‐3‐ O ‐myristate. These pentacyclic terpenoids have been quantied simultaneously using reversed‐phase HPLC with isocratic elution and internal standardisation. Of the 10 varieties of C. ofcinalis investigated, Calypso Orange Florensis produced the highest amounts of the bioactive monoesters, followed by Fiesta Gitana Gelb and May Orange Florensis. The lipophilic extract from the owers of Calypso Orange Florensis variety also contained low levels of the newly characterised calenduladiol‐3‐ O ‐laurate. Copyright © 2004 John Wiley & Sons, Ltd.