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Qualitative analysis and HPLC isolation and identification of procyanidins from vicia faba
Author(s) -
Merghem Rachid,
Jay Maurice,
Brun Nathalie,
Voirin Bernard
Publication year - 2004
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.731
Subject(s) - chemistry , proanthocyanidin , phloroglucinol , chromatography , high performance liquid chromatography , trimer , sephadex , catechin , vicia faba , organic chemistry , polyphenol , botany , dimer , antioxidant , enzyme , biology
Abstract The soluble proanthocyanidins of the coloured seed coats of Vicia faba L. were isolated and separated by solvent partition. The chemical characteristics of the proanthocyanidins were elucidated by total oxidation and partial degradation in the presence of phloroglucinol followed by HPLC analysis. The native extract of proanthocyanidins contained (+)‐gallocatechin, (−)‐epigallocatechin, (+)‐catechin and (−)‐epicatechin units. Oligomeric procyanidins were puried by chromatography on Sephadex LH‐20 and the accessible compounds were isolated by RP‐HPLC using a Licrospher Li 100 Column. The structures of the puried oligomeric procyanidins were elucidated using a procedure involving TLC, UV spectroscopy, ESI‐MS and HPLC analysis of the products from the phloroglucinol reaction. The major condensed tannins of Vicia faba comprise six compounds identied as two A‐type procyanidin dimers, the procyanidin dimers B1, B2 and B3, and a procyanidin trimer. Copyright © 2004 John Wiley & Sons, Ltd.