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Quantification of sideroxylonals in Eucalyptus foliage by high‐performance liquid chromatography
Author(s) -
Wallis Ian R.,
Herlt Anthony J.,
Eschler Bart M.,
Takasaki Midori,
Foley William J.
Publication year - 2003
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.728
Subject(s) - chemistry , chromatography , phloroglucinol , extraction (chemistry) , acetone , high performance liquid chromatography , yield (engineering) , sawdust , organic chemistry , materials science , metallurgy
This paper describes the extraction and quantication of sideroxylonals, a group of formylated phloroglucinol compounds found in the foliage of some eucalypt species. Samples of dry, ground foliage were Soxhlet‐extracted with light petroleum spirit:acetone (4:1) and the resultant extract analysed (in the presence of internal standard) by reversed‐phase HPLC without further purication. The yield of sideroxylonals was exponential with time and showed an inection at ca. 4 h of extraction. It is recommended that samples be extracted for 6 h, giving a 92% recovery of the sideroxylonals. The title compounds deteriorate under various conditions, e.g. 10% are lost when foliage is oven‐dried at 40C compared to freeze‐drying. Storing samples in mobile phase led to a slow deterioration of sideroxylonals with a 7% loss after 4 days, while 22% of these compounds were lost from dry, ground eucalypt leaf stored at room temperature for 20 months. Copyright © 2003 John Wiley & Sons, Ltd.