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The simultaneous determination of 1‐aminocyclopropane‐1‐carboxylic acid and cyclopropane‐1,1‐dicarboxylic acid in Lycopersicum esculentum by high‐performance liquid chromatography—electrospray tandem mass spectrometry
Author(s) -
Petritis Konstantinos,
Koukaki Georgia,
Koussissi Elisabet,
Elfakir Claire,
Dreux Michel,
Dourtoglou Vassilis
Publication year - 2003
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.725
Subject(s) - chemistry , electrospray , chromatography , cyclopropane , tandem mass spectrometry , mass spectrometry , tandem , liquid chromatography–mass spectrometry , carboxylic acid , organic chemistry , ring (chemistry) , materials science , composite material
Varying concentrations of cyclopropane‐1,1‐dicarboxylic acid (CDA), an inhibitor of 1‐aminocyclopropane‐1‐carboxylic acid oxidase, added to the solid culture medium of tomato nodal shoot segments resulted in a reduction in the level of endogenous ethylene according to the concentration of inhibitor applied. Following treatment with inhibitor, plants were homogenised and the concentrations of CDA and of 1‐aminocyclopropane‐1‐carboxylic acid (ACC) were measured simultaneously in the resulting juice using an HPLC‐ESI/MS‐MS method. The levels of CDA and ACC measured in the plant tissues were associated with the concentration of inhibitor added to the solid medium. The HPLC‐ESI/MS‐MS method described produced limits of detection of 0.8 pmol for ACC and of 4 pmol for CDA. Copyright © 2003 John Wiley & Sons, Ltd.