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Determination of the triacylglycerol composition of coffee beans by reverse‐phase high‐performance liquid chromatography
Author(s) -
Jham Gulab N.,
NikolovaDamyavova Boryana,
Viera Mirtes,
Natalino Ricardo,
Rodrigues Augusto Cezar
Publication year - 2003
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.721
Subject(s) - chemistry , chromatography , gradient elution , high performance liquid chromatography , dichloromethane , acetonitrile , composition (language) , green coffee , food science , solvent , biochemistry , linguistics , philosophy
Abstract Reverse‐phase HPLC with refractive index and light scattering detectors in isocratic and gradient elution modes, respectively, was applied for the separation of the major triacylglycerols (TAG) in coffee lipids. Twelve TAG species could be identied and determined using a linear gradient of acetonitrile in dichloromethane: dichloroethane. The quantitative evaluation was based on the relative area percentages derived directly from a data‐station. The procedure was applied to determine the TAG composition of three types of coffee beans harvested in two coffee producing areas in Brazil and dried by two commonly used procedures. No signicant differences in the TAG compositions due to the type, origin and drying procedure were found. Copyright © 2003 John Wiley & Sons, Ltd.