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Analysis of anthraquinones in cell cultures of Cinchona ‘Robusta’ by HPLC with photodiode array and mass spectrometry detection
Author(s) -
Han YingShan,
Hofte Bertil,
der Heijden Robert van,
Verpoorte Robert
Publication year - 2003
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.719
Subject(s) - chemistry , anthraquinones , chromatography , mass spectrometry , photodiode , high performance liquid chromatography , analytical chemistry (journal) , botany , optoelectronics , biology , physics
An HPLC‐PAD‐ESI/MS method has been developed for the analysis of anthraquinones in cell cultures of Cinchona ‘Robusta’. Using a C 18 column and gradient elution with a mobile phase system containing acetonitrile, water and triuoroacetic acid, a satisfactory separation of both anthraquinone glycosides and aglycones in a crude dichloromethane extract could be obtained. Robustaquinone B was identied as a major anthraquinone in the extracts, and another ve anthraquinones were tentatively identied from spectroscopic data. A number of minor unknown compounds were detected and were distinguished from the known anthraquinones. An isocratic system for the quantitative determination of robustaquinone B has also been developed. Copyright © 2003 John Wiley & Sons, Ltd.