Analysis of anthraquinones in cell cultures of  Cinchona  ‘Robusta’ by HPLC with photodiode array and mass spectrometry detection | Zendy

Han YingShan | Zendy; Hofte Bertil | Zendy; der Heijden Robert van | Zendy; Verpoorte Robert | Zendy

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Analysis of anthraquinones in cell cultures of Cinchona ‘Robusta’ by HPLC with photodiode array and mass spectrometry detection

Author(s) -

Han YingShan,

Hofte Bertil,

der Heijden Robert van,

Verpoorte Robert

Publication year - 2003

Publication title -

phytochemical analysis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.574

H-Index - 72

eISSN - 1099-1565

pISSN - 0958-0344

DOI - 10.1002/pca.719

Subject(s) - chemistry , anthraquinones , chromatography , mass spectrometry , photodiode , high performance liquid chromatography , analytical chemistry (journal) , botany , optoelectronics , biology , physics

An HPLC‐PAD‐ESI/MS method has been developed for the analysis of anthraquinones in cell cultures of Cinchona ‘Robusta’. Using a C 18 column and gradient elution with a mobile phase system containing acetonitrile, water and triuoroacetic acid, a satisfactory separation of both anthraquinone glycosides and aglycones in a crude dichloromethane extract could be obtained. Robustaquinone B was identied as a major anthraquinone in the extracts, and another ve anthraquinones were tentatively identied from spectroscopic data. A number of minor unknown compounds were detected and were distinguished from the known anthraquinones. An isocratic system for the quantitative determination of robustaquinone B has also been developed. Copyright © 2003 John Wiley & Sons, Ltd.

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