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Identification of major metabolites in Aloe littoralis by high‐performance liquid chromatography–nuclear magnetic resonance spectroscopy
Author(s) -
Karagianis Gloria,
Viljoen Alvaro,
Waterman Peter G.
Publication year - 2003
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.714
Subject(s) - chemistry , high performance liquid chromatography , chromatography , heteronuclear molecule , nuclear magnetic resonance spectroscopy , heteronuclear single quantum coherence spectroscopy , metabolite , analytical chemistry (journal) , nuclear magnetic resonance , stereochemistry , biochemistry , physics
Examination of the leaf exudate of the South African species Aloe littoralis by reversed‐phase HPLC revealed the presence of two major metabolites. The identication of the two compounds without isolation was attempted by HPLC‐NMR based on separation using a C 18 column eluting with a deuterium oxide:acetonitrile solvent gradient and an inverse HPLC‐NMR probe. For each compound, one‐dimensional proton spectra, and two‐dimensional homonuclear COSY and TOCSY, and heteronuclear HSQC and HMBC, spectra were collected. On the basis of the data obtained, the metabolites were characterised as 10‐hydroxyaloin B and deacetyllittoraloin. Copyright © 2003 John Wiley & Sons, Ltd.