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Fractionation of grape tannins and analysis by matrix‐assisted laser desorption/ionisation time‐of‐flight mass spectrometry
Author(s) -
Perret Camille,
Pezet Roger,
Tabacchi Raphael
Publication year - 2003
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.705
Subject(s) - chemistry , fractionation , proanthocyanidin , chromatography , mass spectrometry , methanol , time of flight mass spectrometry , desorption , reagent , polyphenol , organic chemistry , ionization , adsorption , antioxidant , ion
Abstract Polymeric tannins, extracted from grape berries (Gamay variety), were fractionated according to their mean degree of polymerisation (mDP) on a styrene–divinylbenzene phase eluted with a gradient of methanol:chloroform. Increasing the percentage of methanol led to the solubilisation of higher molecular weight tannins. The mean mDP of each collected fraction was determined by acid‐catalysed degradation in the presence of a nucleophilic reagent. The fractionation method produced a linear gradient of mDP varying between 1.84 and 19.34. The fractions were partially characterised by matrix assisted laser desorption/ionisation time‐of‐flight mass spectrometry (MALDI‐TOF/MS). The spectra showed a complex mixture of proanthocyanidins and galloylated proanthocyanidins up to 4000 amu. Copyright © 2003 John Wiley & Sons, Ltd.