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Chemiluminescence inhibition assay for folic acid using flow injection analysis
Author(s) -
Song Zhenghua,
Wang Lin
Publication year - 2003
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.704
Subject(s) - chemistry , chemiluminescence , potassium ferricyanide , ferricyanide , folic acid , detection limit , luminol , flow injection analysis , chromatography , potassium ferrocyanide , relative standard deviation , ferrocyanide , quantitative analysis (chemistry) , inorganic chemistry , electrode , medicine
A new flow injection method for the determination of folic acid is described. A fast oxidation reaction occurred when folic acid was mixed with potassium ferricyanide generating ferrocyanide which then inhibited the chemiluminescent reaction of ferricyanide and luminol in alkaline medium. The decrease of chemiluminescence intensity was correlated with the folic acid concentration in the range 0.1–21 µg/mL; the detection limit for the assay was 0.03 µg/mL (3σ). A complete analysis of folic acid, including sampling and washing, could be performed within 2 min with a relative standard deviation of less than 4.0%. The proposed method has been applied successfully to the determination of folic acid in pharmaceutical preparations Copyright © 2003 John Wiley & Sons, Ltd.