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Purification of anthocyanins from species of Banksia and Acacia using high‐voltage paper electrophoresis
Author(s) -
Asenstorfer Robert E.,
Morgan Anne L.,
Hayasaka Yoji,
Sedgley Margaret,
Jones Graham P.
Publication year - 2003
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.696
Subject(s) - chemistry , acacia , banksia , electrophoresis , botany , chromatography , biology , woodland
Abstract A new method has been developed for the isolation and rapid identification of anthocyanins from two floricultural crops based on the use of high‐voltage paper electrophoresis with bisulphite buffer. Using this method, anthocyanin pigments were successfully purified as their negatively charged bisulphite‐addition compounds from crude extracts of plant tissue. In conjunction with liquid chromatography–electrospray mass spectrometry, the method enabled the anthocyanins from the flowers of two Banksia species and the leaves of two Acacia species to be identified. The Banksia flowers contained both cyanidin and peonidin‐based pigments, while the Acacia leaves contained cyanidin and delphinidin derivatives. Copyright © 2003 John Wiley & Sons, Ltd.