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Identification of lipophilic flavones and flavonols by comparative HPLC, TLC and UV spectral analysis
Author(s) -
Greenham Jenny,
Harborne Jeffrey B.,
Williams Christine A.
Publication year - 2003
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.693
Subject(s) - flavonols , flavones , chemistry , hydroxylation , chromatography , luteolin , apigenin , organic chemistry , high performance liquid chromatography , flavonoid , antioxidant , enzyme
The identification of lipophilic flavones and flavonols using a combination of high performance liquid chromatography, thin layer chromatography and UV spectral analysis is discussed. Data are provided for the flavones, apigenin, luteolin and tricetin and twelve of their methyl ethers, 8‐hydroxyluteolin, 6‐hydroxyluteolin and scutellarein and fourteen of their methyl ethers, and some 6,8‐dihydroxyapigenin and 6,8‐dihydroxyluteolin derivatives. Data for some forty two flavonols with extra 6‐ and/or 8‐hydroxylation, mostly 6‐hydroxykaempferol and quercetagetin derivatives, are also presented. The remaining compounds analysed include fourteen 5‐deoxyflavones, four 5‐methoxyflavones and five 5‐deoxyflavonols plus further 5‐hydroxylated flavones and flavonols without B‐ring oxidation or with 2′‐, 5′‐ or 6′‐hydroxylation. Copyright © 2003 John Wiley & Sons, Ltd.