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On‐line identification of unstable catalpol derivatives from Jamesbrittenia fodina by LC‐MS and LC‐NMR
Author(s) -
Cogne A.L.,
Queiroz E. F.,
Wolfender J.L.,
Marston A.,
Mavi S.,
Hostettmann K.
Publication year - 2003
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.689
Subject(s) - chemistry , catalpol , glycoside , nmr spectra database , proton nmr , chromatography , cinnamic acid , scrophulariaceae , methanol , carbon 13 nmr , organic chemistry , stereochemistry , spectral line , botany , astronomy , biology , physics
LC‐UV‐MS analysis of the methanol extract of Jamesbrittenia fodina O. M. Hilliard (Scrophulariaceae) revealed the presence of cinnamic ester derivatives. Two isomeric pairs of these constituents were detected, but could not be isolated. In order to identify these unstable compounds, LC‐ 1 H‐NMR spectra were obtained for each individual isomer and standard NMR measurements were performed in‐mixture. The spectra clearly demonstrated that the fractions consisted of mixtures of cis and trans cinnamoyl catalpol glycoside esters. Copyright © 2003 John Wiley & Sons, Ltd.