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Extraction of phyllanthusols A and B from Phyllanthus acidus and analysis by capillary electrophoresis
Author(s) -
Durham D. G.,
Reid R. G.,
Wangboonskul J.,
Daodee S.
Publication year - 2002
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.668
Subject(s) - chemistry , chromatography , capillary electrophoresis , phyllanthus , micellar electrokinetic chromatography , repeatability , extraction (chemistry) , electrophoresis , detection limit , botany , biology
Extracts of roots Phyllanthus acidus were examined by free zone capillary electrophoresis, micellar electrokinetic chromatography (MEKC), and MEKC using the sweeping technique which involves application of a negative potential to the inlet end of the capillary and very much longer than conventional injection times. The latter technique, using a buffer of 50 m M sodium dihydrogen phosphate (pH 2) containing 80 m M sodium dodecylsulphate and 30% methanol was found to allow complete resolution of the active constituents of P. acidus , phyllanthusols A and B, from each other and from other extracted components in under 30 min. Several other components could be detected when hydrodynamic injection times of 500 s were used. The separation, combined with an appropriate extraction procedure and using an internal standard of proguanil, permitted quantification of both phyllanthusols. Calibrations were linear over the range 2–8 µg/mL for phyllanthusol A, and 1–4 µg/mL for phyllanthusol B. Within‐day and day‐to‐day repeatability RSDs were below 10%, and the precision of extraction RSD was around 14%. The limits of quantification and detection were 0.55 and 0.24 µg/mL, respectively. Copyright © 2002 John Wiley & Sons, Ltd.