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LC‐NMR applied to the characterisation of cardiac glycosides from three micropropagated Isoplexis species
Author(s) -
Gavidia Isabel,
Seitz H. Ulrich,
PérezBermúdez Pedro,
Vogler Bernhard
Publication year - 2002
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.655
Subject(s) - chemistry , digitoxigenin , glycoside , pregnane , cardenolide , stereochemistry , digitalis , botany , biology , medicine , heart failure
Species of the genus Isoplexis are of particular interest with respect to the biochemical pathway leading to the cardenolides. It is important to determine whether or not 5β‐configured compounds, typically produced by Digitalis species and used in medicine, are present together with their respective α‐isomers in Isoplexis spp. Structure elucidation by LC‐NMR of the products isolated from in vitro regenerated Isoplexis canariensis, I. chalcantha and I. isabelliana was carried out, and similarities were observed among the products of the three species, including the presence of digitoxigenin‐type cardenolides in I. canariensis and xysmalogenin and canarigenin derivatives in I. chalcantha never previously reported in these species. Pregnane glycosides not found until now either in Isoplexis or Digitalis were also detected. Copyright © 2002 John Wiley & Sons, Ltd.