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HPLC analysis of fusaric acid, 9,10‐dehydrofusaric acid and their methyl esters, toxic metabolites from weed pathogenic Fusarium species
Author(s) -
Amalfitano Carmine,
Pengue Raffaele,
Andolfi Anna,
Vurro Maurizio,
Zonno Maria C.,
Evidente Antonio
Publication year - 2002
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.648
Subject(s) - fusaric acid , chemistry , fusarium , high performance liquid chromatography , fusarium oxysporum , chromatography , strain (injury) , methanol , quantitative analysis (chemistry) , botany , organic chemistry , biology , anatomy
A simple and rapid HPLC method, using a high‐density C 18 column, has been developed for the quantitative analysis of fusaric and dehydrofusaric acids and their methyl esters in the methanol extract of lyophilised culture filtrates of species of Fusarium . The method has been used to determine the content of these metabolites in two strains of Fusarium oxysporum and in strains of F. nygamai and F. udum . Fusaric acid has been isolated and identified from a strain of F. udum for the first time. Copyright © 2002 John Wiley & Sons, Ltd.