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Quantitative determination of oleane derivatives in Terminalia arjuna by high performance thin layer chromatography
Author(s) -
Singh D. V.,
Verma R. K.,
Gupta M. M.,
Kumar Sushil
Publication year - 2002
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.643
Subject(s) - chemistry , terminalia arjuna , chromatography , chloroform , vanillin , thin layer chromatography , high performance thin layer chromatography , methanol , analyte , elution , silica gel , spots , bark (sound) , terminalia , organic chemistry , traditional medicine , medicine , physics , acoustics
Abstract A simple, precise and rapid high performance thin layer chromatographic method has been developed for the simultaneous quantitative determination of five oleane derivatives, namely, arjunic acid, arjunolic acid, arjungenin, arjunetin and arjunglucoside I from stem bark extract of Terminalia arjuna . The isolation and separation of these compounds was carried out on 60 F 254 layers eluted with chloroform:methanol (90:10), and the analytes were visualised through colour development with vanillin in concentrated sulphuric acid:ethanol. Scanning and quantification of the spots at 640 nm showed good recoveries in the range 96.40–101.7%. Copyright © 2002 John Wiley & Sons, Ltd.