Quantitative determination of oleane derivatives in  Terminalia arjuna  by high performance thin layer chromatography | Zendy

Singh D. V. | Zendy; Verma R. K. | Zendy; Gupta M. M. | Zendy; Kumar Sushil | Zendy

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Quantitative determination of oleane derivatives in Terminalia arjuna by high performance thin layer chromatography

Author(s) -

Singh D. V.,

Verma R. K.,

Gupta M. M.,

Kumar Sushil

Publication year - 2002

Publication title -

phytochemical analysis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.574

H-Index - 72

eISSN - 1099-1565

pISSN - 0958-0344

DOI - 10.1002/pca.643

Subject(s) - chemistry , terminalia arjuna , chromatography , chloroform , vanillin , thin layer chromatography , high performance thin layer chromatography , methanol , analyte , elution , silica gel , spots , bark (sound) , terminalia , organic chemistry , traditional medicine , medicine , physics , acoustics

A simple, precise and rapid high performance thin layer chromatographic method has been developed for the simultaneous quantitative determination of five oleane derivatives, namely, arjunic acid, arjunolic acid, arjungenin, arjunetin and arjunglucoside I from stem bark extract of Terminalia arjuna . The isolation and separation of these compounds was carried out on 60 F 254 layers eluted with chloroform:methanol (90:10), and the analytes were visualised through colour development with vanillin in concentrated sulphuric acid:ethanol. Scanning and quantification of the spots at 640 nm showed good recoveries in the range 96.40–101.7%. Copyright © 2002 John Wiley & Sons, Ltd.

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