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Development of an analytical method for the determination of betaines in higher plants by capillary electrophoresis at low pH
Author(s) -
Zhang Jinghua,
Nishimura Naoki,
Okubo Akira,
Yamazaki Sunao
Publication year - 2002
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.639
Subject(s) - chemistry , trigonelline , betaine , capillary electrophoresis , electropherogram , chromatography , phenacyl , electrolyte , glycine , brine , analytical chemistry (journal) , organic chemistry , biochemistry , amino acid , electrode
Abstract In recent years the role of betaines in higher plants has been extensively investigated in relation to environmental stress response. This paper reports the establishment of a simple, rapid and reliable method for the determination of betaines using capillary electrophoresis (CE) at low pH. Betaines were first converted to their phenacyl esters and the crude reaction mixture was then applied directly to CE without any pre‐treatment. Employing an electrolyte running solution at pH 3 gave a well‐resolved electropherogram for the esters of glycine betaine ( 1 ), trigonelline, proline betaine, γ‐butyro betaine, and carnitine. The content of 1 and its variation in plant samples collected from high‐salinity areas in north China and west Australia are presented. Copyright © 2002 John Wiley & Sons, Ltd.