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The extraction of imperialine and imperialine‐3β‐glucoside from Fritillaria pallidiflora Schrenk and quantitative determination by HPLC‐evaporative light scattering detection
Author(s) -
Li Ping,
Zeng Lingjie,
Li Songlin,
Lin Ge
Publication year - 2002
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.635
Subject(s) - chemistry , chromatography , chromatography detector , high performance liquid chromatography , extraction (chemistry) , elution
The extraction procedure and quantitative determination by HPLC‐evaporative light scattering detection (ELSD) of the main bioactive components, namely, imperialine (1) and imperialine‐3β‐glucoside (2), of bulbs of Fritillaria pallidiflora Schrenk have been investigated. The most efficient method for the simultaneous extraction of 1 and 2 involved pre‐treatment of the bulb powder with ammonia, followed by reflux with dichloromethane:methanol at 90°C for 4 h. Simultaneous determination of non‐chromophoric 1 and 2 by HPLC‐ELSD employed a Kromasil™ C 18 column eluted with acetonitrile:water:diethylamine. The assay was accurate and reproducible with an overall variation lower than 4% and a sample recovery higher than 98%. The methods described have been successfully used to evaluate the quality of three batches of the crude traditional Chinese medicinal herb derived from the bulbs of F. pallidiflora . Copyright © 2002 John Wiley & Sons, Ltd.