Premium
Determination of the triterpenoid, betulinic acid, in Doliocarpus schottianus by HPLC
Author(s) -
Oliveira Brás H. de,
Santos Cid A. M.,
Espíndola Ana Paula D. M.
Publication year - 2002
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.628
Subject(s) - betulinic acid , chemistry , chromatography , high performance liquid chromatography , metabolite , bark (sound) , terpene , betulin , elution , organic chemistry , biochemistry , genetics , physics , acoustics , biology
The isolation, detection and quantification of betulinic acid in Doliocarpus schottianus are described. The isolation from the plant extract was made by column chromatography and centrifugal TLC, and betulinic acid was characterized by spectrometric methods. The detection and quantification were made by HPLC using a C 18 column eluted with acetonitrile:water and detected at 210 nm. The results showed that the metabolite accumulates in the bark of the plant, but very small concentrations were also found in the leaves and wood. Copyright © 2002 John Wiley & Sons, Ltd.