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Identification and quantification of galloyl derivatives, flavonoid glycosides and anthocyanins in leaves of Pistacia lentiscus L.
Author(s) -
Romani A.,
Pinelli P.,
Galardi C.,
Mulinacci N.,
Tattini M.
Publication year - 2002
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.627
Subject(s) - chemistry , pistacia lentiscus , glycoside , flavonoid , chromatography , traditional medicine , botany , stereochemistry , biochemistry , ecology , medicine , mediterranean climate , biology , antioxidant
Abstract Separation, identification and quantification of polyphenols was carried out on leaves of Pistacia lentiscus L., an evergreen member of the family Anacardiaceae, using semi‐preparative HPLC, HPLC‐photodiode array detection and HPLC‐MS analysis, together with 1 H‐ and 13 C NMR. Three major classes of secondary metabolites were detected: (i) gallic acid and galloyl derivatives of both glucose and quinic acid; (ii) flavonol glycosides, i.e. myricetin and quercetin glycosides; and (iii) anthocyanins, namely delphinidin 3‐ O ‐glucoside and cyanidin 3‐ O ‐glucoside. Low amounts of catechin were also detected. The concentration of galloyl derivatives was extremely high, representing 5.3% of the leaf dry weight, and appreciable amounts of myricetin derivatives were also detected (1.5% on a dry weight basis). These findings may be useful in establishing a relationship between the chemical composition of the leaf extract and the previously reported biological activity of P. lentiscus , and may also assign a new potential role of P. lentiscus tissue extracts in human health care. Copyright © 2002 John Wiley & Sons, Ltd.