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Quantitative determination of cytotoxic Friedo‐nor ‐oleanane derivatives from five morphological types of Maytenus ilicifolia (celastraceae) by reverse‐phase high‐performance liquid chromatography
Author(s) -
Filho Waldemar Buffa,
Corsino Joaquim,
Bolzani Vanderlan da Silva,
Furlan Maysa,
Pereira Ana Maria S.,
França Suzelei Castro
Publication year - 2002
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.626
Subject(s) - celastraceae , chemistry , chromatography , high performance liquid chromatography , celastrol , cytotoxic t cell , apoptosis , biochemistry , in vitro , stereochemistry
Five different morphological types of Maytenus ilicifolia of the same age and harvested under the same conditions showed distinct accumulations of some friedo‐nor ‐oleananes. A rapid, sensitive and reliable reverse‐phase HPLC method (employing an external standard) was used for the determination of the cytotoxic triterpenoids, 20α‐hydroxymaytenin, 22β‐hydroxymaytenin, maytenin, celastrol and pristimerin in each of the five types. Well resolved peaks with good detection response and linearity in the range1.0–100 µg/mL were obtained. Copyright © 2002 John Wiley & Sons, Ltd.