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Separation of bisbenzylisoquinoline alkaloids by micellar electrokinetic chromatography
Author(s) -
Kuo ChingHua,
Sun ShaoWen
Publication year - 2002
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.624
Subject(s) - chemistry , micellar electrokinetic chromatography , chromatography , electrokinetic phenomena , sodium cholate , lipophilicity , sodium , elution , high performance liquid chromatography , resolution (logic) , analyte , detection limit , organic chemistry , artificial intelligence , computer science
The micellar electrokinetic chromatographic (MEKC) separation of seven bisbenzylisoquinoline alkaloids has been developed. The effects of various separating factors were studied. Optimum separation was achieved using a buffer (pH 9.2) of 20 m M sodium borate and 20 m M sodium dihydrogen phosphate buffer containing 55 m M sodium cholate; the optimum voltage and injection time were 21 kV and 0.05 min, respectively. Highest peak efficiency was obtained when the analytes were dissolved in 10 m M sodium dodecyl sulphate as sample matrix for injection. The elution order of the bisbenzylisoquinoline alkaloids was related to their lipophilicity. The resolution, run time and detection limits of the MEKC method were compared with those of an HPLC method developed previously. Copyright © 2002 John Wiley & Sons, Ltd.