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Liquid chromatography–Atmospheric pressure chemical ionisation/Mass spectrometry: a rapid and selective method for the quantitative determination of ginkgolides and bilobalide in ginkgo leaf extracts and phytopharmaceuticals
Author(s) -
Jensen Anette Gemal,
Ndjoko Karine,
Wolfender JeanLuc,
Hostettmann Kurt,
Camponovo Fabrizio,
Soldati Fabio
Publication year - 2002
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.614
Subject(s) - ginkgolides , chemistry , chromatography , atmospheric pressure chemical ionization , mass spectrometry , ginkgo , terpene , liquid chromatography–mass spectrometry , ginkgo biloba , high performance liquid chromatography , chemical ionization , ionization , organic chemistry , ion , medicine , pharmacology , traditional medicine
Abstract In order to evaluate the composition of active constituents in phytopharmaceutical preparations, valid analytical methods are required. For the determination of the active terpene constituents of Ginkgo biloba (the ginkgolides and bilobalide), a liquid chromatography–mass spectrometry (LC‐MS) method has been developed using atmospheric pressure chemical ionisation (APCI) in the negative ion mode. This detection mode was found to be much more sensitive and selective compared to UV; indeed the ginkgo terpene trilactones lack strong UV chromophores and flavonoids interfere with their UV detection. LC‐APCI/MS detection allowed a considerable reduction in analysis time when compared to LC‐UV, because LC resolution was only needed between the pair of isomers ginkgolide B and ginkgolide J. All compounds were selectively detected by single ion monitoring of their specific deprotonated molecules [M‐H] − . The samples were directly injected without pre‐purification, and a fast gradient was applied, reducing the total time of analysis to 14 min. With this method, the ginkgo terpene trilactones were detected on‐line in the picogram range. Several commercial ginkgo preparations on the Swiss market were analysed, and the ginkgolide and bilobalide contents were evaluated using the method described. Copyright © 2001 John Wiley & Sons, Ltd.