Premium
Age and space distributions of monoterpenes in fresh needles of Picea abies (L) Karst. determined by gas chromatography‐mass spectrometry
Author(s) -
Holubová V.,
Hrdlic̆ka P.,
Kubán̆ V.
Publication year - 2001
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.591
Subject(s) - chemistry , camphene , picea abies , gas chromatography , chromatography , alpha pinene , limonene , gas chromatography–mass spectrometry , mass spectrometry , karst , monoterpene , botany , essential oil , organic chemistry , paleontology , biology
Monoterpenes (α‐ and β‐pinene, δ‐3‐carene, camphene, α‐phellandrene and limonene) were determined by Gas Chromatography‐Mass Spectrometry in fresh needles of Picea abies (L) Karst. situated in three ecologically different regions of Moravia. Through the use of cryogenic grinding for critical sample homogenisation, solvent extraction with cold n ‐hexane, followed by GC analysis with mass detection, very low quantities of sample (0.1–0.3 g needles) could be processed, thus permitting a comparison of amounts of monoterpenes in needles of different ages and a determination of changes in concentrations of monoterpenes in needles at different locations on the tree. The amount of α‐phellandrene decreased with the age of the needles, and the content of δ‐3‐carene was higher in apical branches compared to lateral ones. Copyright © 2001 John Wiley & Sons, Ltd.