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An improved method for the analysis of sennosides in Cassia angustifolia by high‐performance liquid chromatography
Author(s) -
Bala Shashi,
Uniyal G. C.,
Dubey Toshi,
Singh S. P.
Publication year - 2001
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.586
Subject(s) - chemistry , chromatography , resolution (logic) , high performance liquid chromatography , cassia , solvent , chromatography detector , analytical chemistry (journal) , organic chemistry , medicine , alternative medicine , traditional chinese medicine , pathology , artificial intelligence , computer science
A reversed‐phase column liquid chromatographic method for the analysis of sennosides A and B present in leaf and pod extracts of Cassia angustifolia has been developed using a Symmetry C 18 column and a linear binary gradient profile. The method can be utilised for the quantitative determination of other sennosides as a baseline resolution for most of the constituents was achieved. The method is economical in terms of the time taken and the amount of solvent used (25 mL) for each analysis. The validity of the method with respect to analysis was confirmed by comparing the UV spectra of each peak with those of reference compounds using a photodiode array detector. Copyright © 2001 John Wiley & Sons, Ltd.