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Simultaneous determination of (+)‐catechin and (−)‐epicatechin in cacao and its products by high‐performance liquid chromatography with electrochemical detection
Author(s) -
Subagio Achmad,
Sari Puspita,
Morita Naofumi
Publication year - 2001
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.583
Subject(s) - chemistry , chromatography , high performance liquid chromatography , catechin , resolution (logic) , detection limit , quantitative analysis (chemistry) , methanol , extraction (chemistry) , solid phase extraction , polyphenol , organic chemistry , antioxidant , artificial intelligence , computer science
A fast single‐step extraction procedure and HPLC with electrochemical detection (ECD) in the oxidative mode has been used for the simultaneous, quantitative determination of (+)‐catechin (CA) and (−)‐epicatechin (EC) in cacao and its products. A UV detector measuring at 280 nm was also used in‐line for comparison purposes. The samples were extracted with methanol and applied directly to the reversed‐phase column of the HPLC. The high sensitivity of the ECD allowed operation at a low voltage (+0.5 V), but with a high sensitivity for the readily oxidisable CA and EC, thus reducing baseline noise. As a result, ECD had a better sensitivity and resolution than UV detection for the simultaneous quantitative determination of CA and EC in cacao and its products. Copyright © 2001 John Wiley & Sons, Ltd.