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A simple, rapid method for HPLC analysis of lycopene isomers
Author(s) -
Ishida Betty K.,
Ma Jason,
Chan Bock
Publication year - 2001
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.576
Subject(s) - chemistry , lycopene , chromatography , elution , high performance liquid chromatography , carotenoid , ether , extraction (chemistry) , resolution (logic) , pigment , methanol , quantitative analysis (chemistry) , cis–trans isomerism , ethyl acetate , organic chemistry , biochemistry , artificial intelligence , computer science
A rapid method for the extraction, separation and quantification of the geometric isomers of lycopene and β‐carotene from tomato fruit is described. Carotenoids in tomato were separated and eluted using a reversed‐phase HPLC with a C 30 column and a mobile phase consisting of methyl‐ t ‐butyl ether, methanol and ethyl acetate. The system provided sharp resolution of cis ‐ and trans ‐isomers of lycopene within approximately 23 min in contrast to the longer and more complex gradient procedures required by previously described methods. Experiments indicate that the stability of extracts of fresh tomato may be improved if stored at −20°C, and that the presence of the antioxidant BHA has no apparent effect on stability. Copyright © 2001 John Wiley & Sons, Ltd.