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Instant determination of the artemisinin from various Artemisia annua L . extracts by LC‐ESI‐MS/MS and their in‐silico modelling and in vitro antiviral activity studies against SARS‐CoV‐2
Author(s) -
Dogan Kubra,
Erol Ebru,
Didem Orhan Muge,
Degirmenci Zehra,
Kan Tugce,
Gungor Aysen,
Yasa Belkis,
Avsar Timucin,
Cetin Yuksel,
Durdagi Serdar,
Guzel Mustafa
Publication year - 2022
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.3088
Subject(s) - artemisinin , artemisia annua , chemistry , artesunate , chromatography , natural product , in silico , protease , liquid chromatography–mass spectrometry , in vitro , tandem mass spectrometry , traditional medicine , pharmacology , plasmodium falciparum , mass spectrometry , biochemistry , enzyme , malaria , medicine , gene , immunology , biology
Numerous efforts in natural product drug development are reported for the treatment of Coronavirus. Based on the literature, among these natural plants Artemisia annua L . shows some promise for the treatment of SARS‐CoV‐2. Objective The main objective of our study was to determine artemisinin content by liquid chromatography electrospray ionisation tandem mass spectrometry (LC‐ESI‐MS/MS), to investigate the in vitro biological activity of artemisinin from the A. annua plants grown in Turkey with various extracted methods, to elaborate in silico activity against SARS‐CoV‐2 using molecular modelling. Methodology Twenty‐one different extractions were applied. Direct and sequential extractions studies were compared with ultrasonic assisted maceration, Soxhlet, and ultra‐rapid determined artemisinin active molecules by LC‐ESI‐MS/MS methods. The inhibition of spike protein and main protease (3CL) enzyme activity of SARS‐CoV‐2 virus was assessed by time resolved fluorescence energy transfer (TR‐FRET) assay. Results Artemisinin content in the range 0.062–0.066%. Artemisinin showed significant inhibition of 3CL protease activity but not Spike/ACE‐2 binding. The 50% effective concentration (EC 50 ) of artemisinin against SARS‐CoV‐2 Spike pseudovirus was found greater than 50 μM (EC 45 ) in HEK293T cell line whereas the cell viability was 94% of the control ( P < 0.01). The immunosuppressive effects of artemisinin on TNF‐α production on both pseudovirus and lipopolysaccharide (LPS)‐induced THP‐1 cells were found significant in a dose dependent manner. Conclusion Further studies of these extracts for COVID‐19 treatment will shed light to seek alternative treatment options. Moreover, these natural extracts can be used as an additional treatment option with medicines, as well as prophylactic use can be very beneficial for patients.