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Structural characterisation and screening of triterpene saponins in the bark of Ilex rotunda using high‐performance liquid chromatography coupled to electrospray ionisation and quadrupole time‐of‐flight mass spectrometry
Author(s) -
Ling Yun,
Zhang Qing
Publication year - 2020
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2986
Subject(s) - chemistry , chromatography , triterpene , quadrupole time of flight , mass spectrometry , electrospray ionization , electrospray , high performance liquid chromatography , formic acid , tandem mass spectrometry , bark (sound) , gradient elution , elution , medicine , alternative medicine , physics , pathology , acoustics
The bark of Ilex rotunda (BIr) has been used as a traditional Chinese medicine for a long time in China. Triterpene saponins (TSs) were considered to be the major bioactive ingredients and possessed antiplatelet aggregation activity. Identification of TSs in BIr is a necessary step to understand the biological properties. Objective This article describes a method for the rapid identification of TSs in BIr. Method An analytical method based on high‐performance liquid chromatography coupled to electrospray ionisation and quadrupole time‐of‐flight tandem mass spectrometry (HPLC‐ESI‐QTOF‐MS/MS) was established to characterise the TSs in BIr. Chromatographic separation was performed on an Agilent poroshell 120 EC‐C18 column with gradient elution using water containing 0.1% ( v / v ) formic acid and acetonitrile as the mobile phase. Results A total of 26 TSs were identified or tentatively identified from BIr. Of them, 23 TSs were first reported from BIr. Conclusion This was the first comprehensive investigation on TSs in BIr, and the results formed the foundation for the further development and utilisation of BIr in the future.