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The combination of HPLC and biological analysis to determine the quality markers and its structural composition of Eclipta prostrata L.
Author(s) -
Wang Long,
Huang Bin,
Li Chenzi,
Yang Bin,
Jia Xiaobin,
Feng Liang
Publication year - 2020
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2969
Subject(s) - chemistry , high performance liquid chromatography , in vivo , chromatography , composition (language) , urine , food science , biochemistry , microbiology and biotechnology , biology , linguistics , philosophy
The diversity and complexity of components are important reasons for the unstable efficacy and safety of Chinese materia medica (CMM) in quality control. The good and stable quality control may be related to the quality markers with structural composition of multi‐components. Objective In the present study, we take Eclipta prostrata L. as a representative example. The 11 samples were collected from the different areas in China, and the discrepancy in bioactivity and chemical composition of these samples were compared. DOX (doxorubicin hydrochloride)‐induced ICR mice were established for in vivo nephrotic syndrome experiments. The biochemical indicators including 24‐h urine protein, triglyceride (TG), etc. were measured and the pathological change of kidney tissue was observed. MPC‐5 cells damage model was induced to compare the difference of these samples in bio‐activity. High‐performance liquid chromatography (HPLC) method for 11 EEP (extract of Eclipta prostrata L.) samples were performed to analyse the content of the quality markers. Results In vivo experiments, EEP could mitigate the content or activity of urine protein, TG, etc. compared with the positive group (TG content was 2.53 ± 0.39 mmol/L, urinary protein quantification on 35th day was 16.79 ± 2.32 mg). In vitro experiments, CCE (coumestans component of Ecliptae) and EEP had equivalent effects on biochemical indicators such as cell viability, etc. According to the HPLC analysis, the content of wedelolactone was 45.88% and demethylwedelolactone was 23.74% in CCE. Conclusion The CCE with a ratio of 2:1 can be considered as a quality marker of Eclipta prostrata L.. This research may provide a perspective for quality control of CMM.