Premium
Kwakhurin‐magnetic particles conjugates enable fast enzyme immunoassay for the detection of kwakhurin in Pueraria candollei
Author(s) -
Sakamoto Seiichi,
Eto Ryota,
Nuntawong Poomraphie,
Yusakul Gorawit,
Juengwatanatrakul Thaweesak,
Putalun Waraporn,
Fujii Shunsuke,
Tanaka Hiroyuki,
Morimoto Satoshi
Publication year - 2020
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2964
Subject(s) - chemistry , immunoassay , chromatography , conjugate , antibody , immunology , medicine , mathematical analysis , mathematics
Kwakhurin (Kwa) is one of the unique isoflavonoids produced in Pueraria candollei var. mirifica ( P. candollei ), which has long been used as folk medicine for rejuvenation in Thailand. Recently, the use of P. candollei ‐derived products has widely spread among Japanese women for cosmetic purposes. Correspondingly, there has been an increase in the number of reports regarding possible health hazards caused by estrogenic activity inherent to the plant; thus, the need for a detailed evaluation of the phytoestrogen content of P. candollei ‐derived products has gained a sense of urgency in recent years. Objective This study aims to develop a rapid enzyme immunoassay that can be applied to the quantitative analysis of Kwa in P. candollei and its derived products. Material and Method A rapid and sensitive immunoassay was developed with a combination of Kwa‐specific monoclonal antibody (MAb 11F) and Kwa–magnetic particles (MPs) conjugates, which increased the surface area of the solid phase, resulting in a decrease in the immunoreaction time. Result This novel MPs‐based enzyme immunoassay (MPs‐EIA) was used to determine Kwa concentration in the range from 2.44 to 78.1 ng/mL with a limit of detection of 1.90 ng/mL. Validation analyses revealed that the proposed MPs‐EIA protocol was sufficiently precise and accurate for effective quantitative analysis of Kwa in P. candollei and its derived products.