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Multi‐phase extraction of ephedrine from Pinellia ternata and herbal medicine using molecular imprinted polymer coated ionic liquid‐based silica
Author(s) -
Fang Luwei,
Xie Jinfeng,
Lin Liangwen,
Tian Minglei,
Row Kyung Ho
Publication year - 2019
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2888
Subject(s) - chemistry , chromatography , molecularly imprinted polymer , ephedrine , extraction (chemistry) , high performance liquid chromatography , solid phase extraction , organic chemistry , selectivity , neuroscience , biology , catalysis
Ephedrine is a typical compound found in lots of plant species that is used in several medicines for the treatment of asthma and bronchitis. However, excess amounts are harmful to humans, so it needs to be removed. Objective This study developed a multi‐phase extraction (MPE) method with a molecular imprinted polymer (MIP) coated ionic liquid (IL)‐based silica (SiO 2 @IL@MIP) to simultaneously extract and separate ephedrine from Pinellia ternata , 10 medicines, and urine samples. Methods IL was immobilized on silica. Subsequently, the IL was combined with the functional monomer, followed by the addition of the crosslinker and template. The resulting sorbent was applied to the MPE, and the extraction, washing and elution solvents were evaluated. Results Fourier‐transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) confirmed the synthesis of SiO 2 @IL@MIP. A maximum adsorption amount of 5.76 mg/g was obtained at 30°C at a neutral pH. In MPE, 10.00 mL of methanol could extract all the ephedrine from Pinellia ternata . The interference was removed by washing with 4.00 mL of water, ethanol, and acetonitrile. Finally, 8.00 mL of methanol/acetic acid (99:1, v / v ) was applied as the elution solvent. The following were extracted: 5.50 μg/g of ephedrine from Pinellia ternata , 0.00–46.50 μg/g from the 10 herbal medicines, and 68.70–102.80 μg/mL in the urine samples. Conclusion The proposed method was applied successfully to the simultaneously extraction and separation of ephedrine from plants and medicines. These results are expected to provide important data for the development of new methods for the separation and purification of bioactive compounds.