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Development of an enzyme immunoassay to detect and quantify psoralen and bergapten in plants
Author(s) -
Bourgaud F.,
Bouque V.,
Marchal S.,
Innocenti G.,
Dall'Acqua F.,
Guckert A.
Publication year - 1995
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2800060607
Subject(s) - psoralen , chemistry , bergapten , chromatography , immunoassay , detection limit , methoxsalen , furanocoumarin , biochemistry , dna , medicine , psoriasis , dermatology , antibody , immunology , biology
A competitive enzyme immunoassay has been developed in order to quantify furanocoumarins. 5‐Hydroxypsoralen was modified and linked to bovine serum albumin to obtain an antigenic response. The rabbit serum that was collected was highly specific to 5‐methoxypsoralen and psoralen but did not recognize angelicin (a psoralen angular isomer), 8‐methoxypsoralen or 5,8‐dimethoxypsoralen, coumarin and related molecules. The detection limit was about 5 ng/mL for 5‐methoxypsoralen and 30 ng/mL for psoralen. The test was applied to plants that contain furanocoumarins i.e. species of Psoralea. The correlation with a classical high‐pressure liquid chromatographic method was satisfactory with both dry and fresh matter. With reference to the extraction method used with the plant material, the detection limit in the plant samples was about 15 μg/g for the dry matter and 20 μg/g for the fresh matter. This technique is a useful tool to detect and quantify psoralen and 5‐methoxypsoralen in plant samples.