Premium
Quantitative determination of podophyllotoxin and related compounds in podophyllum species by reverse phase high performance liquid chromatography
Author(s) -
Bastos Jairo K.,
Kopycki Wlodzimierz J.,
Burandt Charles L.,
Nanayakkara N. P. Dhammika,
McChesney James D.
Publication year - 1995
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2800060207
Subject(s) - chemistry , podophyllotoxin , chromatography , high performance liquid chromatography , gradient elution , elution , reversed phase chromatography , glycoside , stereochemistry
A rapid sensitive and reliable reverse phase high performance liquid chromatography method was developed using a Taxsilr̀ column and gradient elution for the routine determination of podophyllotoxin and six related compounds, epipodophyllotoxin, α‐peltatin, β‐peltatin, 4′‐demethylpodophyllotoxin, podophyllotoxin‐4‐O‐glycoside, and 1,2,3,4‐dehydrodesoxypodophyllotoxin in Podophyllum extracts. The elaborated method gave well resolved peaks for these compounds with good detection response and linearity in the range 25–450 ng.