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Trace analysis of harman alkaloids in passiflora incarnata by reversed‐phase high performance liquid chromatography
Author(s) -
Rehwald Anne,
Sticher Otto,
Meier B.
Publication year - 1995
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2800060206
Subject(s) - chemistry , chromatography , high performance liquid chromatography , peganum harmala , detection limit , alkaloid , solid phase extraction , extraction (chemistry) , botany , stereochemistry , biology
Seventeen different samples of Passiflora incarnata were screened for harman alkaloids by a reversed‐phase high performance liquid chromatographic (HPLC) method. Only one sample yielded a possible harman content of approximately 0.1 ppm which was just above the level of detection limit. The method comprises extraction of the plant material, purification and concentration of the alkaloid fraction by combined solid‐phase extractions on Extrelut and cation‐exchange cartridges, and subsequent HPLC analysis using diode‐array and fluorescence detection. The method was found to be sensitive and selective and revealed good recovery. The efficiency of the method was demonstrated with Peganum harmala seeds.