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Analysis of crude extracts and fractions of brazilian polypodiaceae by high‐resolution gas chromatography‐mass spectrometry. I. triterpenes
Author(s) -
Patitucci Maria Lucia,
Pinto Angelo C.,
Cardoso Jari N.
Publication year - 1995
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2800060106
Subject(s) - chemistry , chromatography , mass spectrometry , terpene , resolution (logic) , phytochemical , rhizome , gas chromatography , gas chromatography–mass spectrometry , high resolution , triterpene , organic chemistry , botany , medicine , biochemistry , alternative medicine , pathology , artificial intelligence , computer science , biology , remote sensing , geology
Characterization of individual components in plant extracts through classical phytochemical methods is a multi‐step procedure which is both costly and time‐consuming. Also significant amounts of extract are required and detection of minor compounds is frequently not possible. The method described in this work involves direct analysis of crude or prefractioned apolar or medium‐polar extracts by high‐resolution gas chromatography and computerized high resolution gas chromatography‐mass spectrometry, followed by co‐injections of the crude extract with certified standards on capillary columns of different polarities. The effectiveness of the method is illustrated for several species of Polypodiaceae (spores and rhizomes). allowing easy monitoring of compound‐type distributions (e.g. triterpenes) and detection of structures present at trace levels (for example, 22,29,30‐trisnorhopane).