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Micellar electrokinetic chromatographic/ultraviolet diode array analysis of Eleutherococcus senticosus
Author(s) -
Pietta P. G.,
Mauri P. L.,
Gardana C.,
Zini L.
Publication year - 1994
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2800050607
Subject(s) - chemistry , chromatography , micellar electrokinetic chromatography , capillary electrophoresis , electrokinetic phenomena , ultraviolet , capillary action , sodium dodecyl sulfate , constant voltage , electrophoresis , analytical chemistry (journal) , voltage , physics , materials science , quantum mechanics , composite material
The main components of the roots of Eleutherococcus senticosus have been separated by capillary electrophoresis in the micellar mode. The best separation was achieved using a fused silica capillary tube and a 25 mM sodium borate buffer (pH 8.25) containing sodium dodecyl sulphate (SDS; 50 mM) at a constant voltage of 280 V/cm. The quantitative determination of eleutheroside B and E in E. senticosus extracts and formulations demonstrates the applicability of this method.