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Rapid high performance liquid chromatographic quantification of valepotriates in hairy root cultures of Valeriana officinalis L. var. sambucifolia Mikan
Author(s) -
Gränicher F.,
Christen P.,
Vuagnat P.
Publication year - 1994
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2800050605
Subject(s) - chemistry , chromatography , high performance liquid chromatography , officinalis , quantitative analysis (chemistry) , chromatographic separation , botany , biology
The quantitative determination of valepotriates in Valeriana officinalis L. var. sambucifolia Mikan hairy roots by a new high performance liquid chromatographic (HPLC) method is reported. The quantification is based on the simultaneous isocratic determination of the monoene and diene derivatives at one wavelength (208 nm). The analysis is rapid and highly reproducible, giving results similar to those obtained with a currently used HPLC method in which valepotriate quantification is undertaken using two separate runs.