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Accurate molecular mass determination by mass spectrometrical techniques: Differentiation of soybean lipoxygenase isoenzymes
Author(s) -
Scheller G.,
Stahl B.,
Weyd S.,
Karas M.,
Hillenkamp F.,
Schreier P.
Publication year - 1994
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2800050602
Subject(s) - chemistry , molecular mass , chromatography , lipoxygenase , isozyme , gel electrophoresis , mass spectrometry , polyacrylamide gel electrophoresis , enzyme , biochemistry
Molecular mass determinations for the four lipoxygenase isoenzymes from soybean seeds were compared using two mass spectrometrical (MS) techniques as well as gel permeation chromatography and sodium dodecylsulphate‐polyacrylamide gel electrophoresis. The data obtained by MS techniques were in good agreement with the molecular masses evaluated from the corresponding cDNA. The MS techniques allowed to distinguish between the lipoxygenase isoenzymes and to check the purity of enzyme preparations.