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Quantitative analysis of alizarin in tissue cultures of Rubia species by high performance liquid chromatography
Author(s) -
Lodhi A. H.,
Sant'Ana A. E. G.,
Charlwood B. V.
Publication year - 1994
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2800050509
Subject(s) - alizarin , chemistry , chromatography , high performance liquid chromatography , alizarin red , acetic acid , elution , gradient elution , callus , botany , nuclear chemistry , organic chemistry , medicine , staining , pathology , biology
A rapid, high performance liquid chromatographic (HPLC) method has been developed for the quantitative analysis of alizarin in field grown roots and tissue cultures of Rubia tinctorum and R. peregrina. Alizarin was quantified by HPLC in the presence of trichlorophenol as internal standard using an RP C‐18 Nova‐pak® column eluted isocratically with methanol: 10% aqueous acetic acid (60:40). The minimum detection limit of alizarin was found to be 10.5 ng. The field grown roots and callus of R. tinctorum contained, respectively, 939.6 (±17.4) and 335.2 (±4.9) μg/g dry weight total alizarin, whilst similar tissues of R. peregrina contained 147.9 (±10.0) and 216.8 (μ0.4) μg/g dry weight total alizarin.