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Separation and quantitation of stevioside and rebaudioside a in plant extracts by normal‐phase high performance liquid chromatography and thin‐layer chromatography: A comparison
Author(s) -
NikolovaDamyanova Boryana,
Bankova Vasya,
Popov Simeon
Publication year - 1994
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2800050207
Subject(s) - stevioside , chemistry , chromatography , stevia rebaudiana , rebaudioside a , thin layer chromatography , densitometry , high performance thin layer chromatography , high performance liquid chromatography , silica gel , resolution (logic) , quantitative analysis (chemistry) , glycoside , diosgenin , chromatography column , organic chemistry , medicine , physics , alternative medicine , food science , pathology , quantum mechanics , artificial intelligence , computer science
Two similar methods, normal phase high performance liquid chromatography and silica gel thin‐layer chromatography with densitometry, were applied for the separation and quantitative determination of the diterpene glycosides stevioside and rebaudioside A in the leaves of the plant Stevia rebaudiana Bertoni. Results were compared and found to be very close in all basic features: resolution, accuracy and reproducibility. Thin‐layer chromatrography was found to be more suitable in case of phytochemical screening of large number of samples.