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Quantitative analysis of gibberellins by isotope dilution mass spectrometry: A comparison of the use of calibration curves, an isotope dilution fit program and arithmetical correction of isotope ratios
Author(s) -
Croker Stephen J.,
Gaskin Paul,
Hedden Peter,
MacMillan Jake,
MacNeil Kenneth A. G.
Publication year - 1994
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2800050206
Subject(s) - isotope dilution , chemistry , mass spectrometry , isotope , chromatography , calibration curve , analytical chemistry (journal) , dilution , gibberellin , sativum , calibration , detection limit , mathematics , botany , physics , statistics , quantum mechanics , biology , thermodynamics
The quantitative analysis of gibberellin A 1 (GA 1 ) in apices of 19‐day‐old seedlings of Pisum sativum L. cultivar Alaska is described using isotope dilution mass spectrometry. Three methods are compared for determining the dilution of isotope in the internal standards, [17‐ 2 H 2 ]GA 1 and [2β‐ 2 H 1 ]GA 1 . Similar results were obtained with each method. The use of calibration curves gave the most precise determination with [ 2 H 2 ]GA 1 as internal standard irrespective of the amount added. The use of an isotope dilution fit program was more precise using [ 2 H 1 ]GA 1 as internal standard. A simpler arithmetical correction procedure gave consistently higher estimates with both internal standards. The relative merits of the methods are discussed and the requirements for reliable quantification by isotope dilution gas chromatography‐mass spectrometry are presented.