Premium
Determination of the sesquiterpene dialdehyde polygodial by high‐pressure liquid chromatography
Author(s) -
van Beek Teris A.,
van Dam Nicole,
De Groot Aede,
Geelen Threes A. M.,
van der Plas Linus H. W.
Publication year - 1994
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2800050106
Subject(s) - chemistry , chromatography , sesquiterpene , high performance liquid chromatography , hexane , extraction (chemistry) , reproducibility , polygonum , solid phase extraction , selectivity , cartridge , organic chemistry , medicine , mechanical engineering , engineering , catalysis , traditional medicine
A high‐pressure liquid chromatographic procedure for the determination of the antifeedant polygodial in Polygonum hydropiper leaves was developed. For leaves, the method consists of a selective extraction with hexane: EtOAc (8:2), pre‐purification of the evaporated extract over a solid phase C2 column, separation with a C18 RP‐HPLC column and detection/quantitation by means of UV at 233 nm. As internal standard α‐ionone is used. Advantages of the method are its selectivity and speed. Various aspects such as extraction efficiency, recovery, peak purity and reproducibility have been evaluated. The minimal detectable quantity is 1 ng.