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Analysis of poppy straw and poppy straw concentrate by reversed‐phase high‐performance liquid chromatography
Author(s) -
GómezSerranillos P.,
Carretero E.,
Villar A.
Publication year - 1994
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.2800050105
Subject(s) - chemistry , poppy , straw , chromatography , botany , inorganic chemistry , biology
A method of reversed‐phase high‐performance liquid chromatography using an elution gradient has been developed to analyse morphine, codeine, thebaine, and oripavine — this last‐mentioned substance for the first time — in samples of poppy straw and poppy straw concentrate. Gradient elution of the octadecylsilane column was performed: the gradient was from 10 to 30% of solvent A (methanol) in solvent B (containing 67% of 0.5% aqueous ammonium acetate solution containing diethylamine, and 33% of methanol) over a 25 min period. The four alkaloids were identified and quantified with the aid of an internal standard.